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  • Niloofar
    Junior Member
    • May 2012
    • 9

    #1

    Hisat2 output is empty

    I run this command:

    hisat2 -p 40 -q -x Ene_index --sra-acc SRR13696274 --al alignedRNA_1 -S alignment1

    and the output says:
    4249510 reads; of these:
    4249510 (100.00%) were unpaired; of these:
    3194713 (75.18%) aligned 0 times
    1045690 (24.61%) aligned exactly 1 time
    9107 (0.21%) aligned >1 times
    24.82% overall alignment rate

    However, the produced alignedRNA_1 file is empty

    Does anyone know what the problem may be?

    Thanks
  • Niloofar
    Junior Member
    • May 2012
    • 9

    #2
    Ok - here's the reply I got from the developers, which solved the problem

    Hi Niloofar,

    Thank you for your reporting.
    Could you download the SRA file (use fastq-dump program from SRAToolkit) and run HISAT2 with the file again?
    There is a problem when you use --sra-acc and --al (--al*, --al-conc* , --un-conc* options) together, HISAT2 doesn't create read files properly. We'll fix it.

    Thanks
    Chanhee

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