How to analyse base mods on personal computer
I would like an opinion on how best to analyse pacbio data for base modifications when there is no access to a sequencing facility running an established SMRT link system. I only need the SMRT tools for this but have not found an easy way to install them on my iMac. There does not seem to be any third party software to do the same thing. Any advice appreciated.
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM