hi, guys, I was wondering what's your take on this. Tophat will accept reads that are mapped up to 20 locations and take a fraction for each location. Well, this could be helpful to identify homologous genes, but also create a lots artifact. Do you think we should stay with reads that mapped only once in the genome?
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Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
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Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
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