is rna-seq experiment strand-specific or not?

[laupl]

hi all,

is there any particular field to search for understand if an rna-seq experiment is or not strand-specific on NCBI SRA db or EMBL ENA db?

thanks

laupl

[malachig]

My impression is that a very small percentage of RNA-seq data generated to date is strand-specific. I am not aware of any way to search for this data directly in SRA. I would be very interested to hear from other users with experience querying SRA. I have found that unless you already know the accession number for the SRA data you are interested in (from a manuscript say) its very difficult to extract meaningful information from the SRA...

[malachig]

Follow up. If you are just looking for some example data that is strand-specific... Both the iDEA Challenge and Human Body Map projects at Illumina include some directional RNA-seq libraries.