Does anyone have any information about Nextera multiplex library prep versus the Illumina method?
-
-
You should be able to find the information you need in the Nextera Illumina-compatible protocol (click the "protocol" link on the product page). Appendix A lists the sequences of our barcode primers; you can also design your own if you wish.
If you have any other questions or issues, feel free to contact me via PM. -
Multiplex prep
We used the Nextera multiplex primers to prepare several bacterial libraries, then pooled the libraries for one lane of Illumina sequencing. This was quite straightforward following the manufacturer's instructions. The libraries sequenced well and we could identify a perfect match to each 6 bp index sequence in 97% of the Illumina index reads. Be aware that if one is performing paired read sequencing, the second of the paired reads (actually Illumina read 3) will have low quality in the last 10-20bp because the index read (read 2) uses some of read 3's reagents. The read1 quality averaged Q20 out to 120 bp while Q20 was around base 110. We found the Nextera kit to be very, very simple and much faster compared to the standard Illumina prep, although we have not examined any potential read bias yet.Last edited by Boonie; 12-30-2010, 11:04 AM.Comment
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Comment