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Old 01-12-2009, 01:53 AM   #1
Eshchar
Junior Member
 
Location: South Africa

Join Date: Nov 2008
Posts: 3
Default mRNA preparation for RNA-Seq

Hi all,

Regarding RNA preparation for mRNA seq (we are using Illumina), we are using Qiagen's Oligotex kit for poly-T selection. So far we have observed that in the final sequencing, approximately 40% of our short reads are ribosomal RNA derived.

Can people perhaps share how much rRNA sequence is present in their Illumina RNA seq runs, and what sort of mRNA selection are they using?

Also, are any users familiar with the of Ribo-minus rRNA depletion kit from Invitrogen?
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