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Has anyone automated any or all of the Illumina library prep steps on a robot? Beckman, Tecan, Hamilton? Any help will be appreciated. Thanks
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Co-ask. I am interested in who is doing it and what advantage can be offered. -
Same here
Looking for Library prep automation to increase throughput and eliminate variations caused by different users. In addition to the automation mentioned above from BIG_SNP, are there any other new instrumentation for full automation, say with thermal blocks?Comment
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SPRIworks Fragment Library System I and II are claimed to be the walk away automates for the library preparation (Beckman Genomics). I would like to know what they actually do, especially the start point (blood?) and endpoint (Adaptor Ligation???) for Illumina libraryComment
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Input is fragmented DNA (not cleaned up), output is adaptor ligated lib. ready for PCR.Originally posted by Michael L. Altshuler View PostComment
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We are currently trying to automate as much of the set up as possible. We are using DNA fragmentase to fragment the DNA and think we can automate a number of the subsequent ligation and adapter annealing steps, although this involves manually placing plates onto PCR blocks. We are going to be using the ampure xp for the PCR clean up steps and dont envisage that being problematic. Finally we are using nimblegen sequence capture in solution and have a protocol for that but we have not run any samples yet. We are going to use a beckman Fx for the pre-PCR steps and a beckman Nx for the post-PCR steps and we are aiming to run a test batch of samples in the next 2 weeks once all our reagents arrive. I'd be happy to pass on details when we have an optimised pipeline.Comment
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Illumina uses Hamilton Robots In house to do their testingComment
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Does anyone actually have the Caliper system up and running in their lab for mRNAseq or DNAseq library construction?Comment
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Sanger have a set up for 96 and 384 well library prep (Beckman, I think).
They also have another setup for Exome capture and prep.
Illumina's robot looked like it would do the job when I saw it at their User Meeting.Comment
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Hi, I can confirm what James said above. The Sanger use a Biomek FX and a Biomek NX to automate their library prep for the Illumina. Beckman also have another site in the UK automating 96 library preps at a time on the Biomek FX with qPCR set up done on a Biomek 3000.Comment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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