[Berkeley2010]

Hi,

I am trying to enrich my pair-end library. I made two libraries 300 bp and 600 bp. At the end PCR step, I get only 26 ng/ul and 15ng/ul for the libraries. This was estimated by nanodrop. I used 10 PCR cycles. How can I get more library. Do I have problems with my adapters that they are not ligated properly. Any help is really appreciated.

[huguesparri]

If your Nanodrop concentration values are acurate, you have enough material to perform a sequencing on many complete Flow Cells for each library.

Nevertheless, I would run both libraries using a DNA1000 Chip on a Bioanalyzer to be sure of the concentration. In our experience, Nanodrop tends to over-estimate concentration values for illumina's libraries.