Homemade illumina oligos and library prep kit

[elly]

Hi,

I am wondering if we can use the PCR amplification primers which were made at our side.
In theory, this can work properly.

But I am worrying a little bit if there can be some problem with sequencing primer binding and PCR amplification step.

Is there anyone who attempt this?

Thank you in advance.

[graveley]

Yes, as long as the sequences you use are the same as the Illumina sequences, primers you synthesize yourself work fine!

[andibody]

Is this also true for adaptors and sequencing primers?

Thanks
Andi

[kkannan]

For the PE PCR primers, is it necessary to PAGE purify them?