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Old 01-13-2011, 02:11 AM   #14
arabidopsis
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Location: Denmark

Join Date: Oct 2010
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I used RiboMinus of Epicentre and got a weird result. RNA concentration after rRNA depletion was very low (predictable), but that low that I could hardly detect it by nanodrop. Does anyone know what rRNA depleted sample from HELA cells should look like (concentration, quality control values etc)? Can you detect RNA concentration after rRNA removal? I got about 20 ng/ul, but measurements are inconsistent and both 260:280 and 240/260 ratios are very low.
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