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  • calexand
    Junior Member
    • Jan 2011
    • 2

    #1

    MAQ .map files to .bed/.sam for MACS

    Hello,

    I would like to use the output .map of a MAQ alignment of unpaired reads as input for the MACS peak caller, and so I want to convert the .map files (binary or txt) to any format recognized by MACS:

    "BED" or "ELAND" or "ELANDMULTI" or "ELANDMULTIPET" or "ELANDEXPORT" or "SAM" or "BAM" or "BOWTIE"

    If you know a tool for it I would appreciate an advice .

    Thank you!
  • calexand
    Junior Member
    • Jan 2011
    • 2

    #2
    I found this command used after mapview, from a tutorial written by Mali Salmon-Divon and Christiana Spyro.
    Weird to answer to myself but perhaps somebody else might find it useful.

    awk '{print $2 "\t" ($3-1) "\t" ($3+25) "\t" "*" "\t" "*" "\t" $4}' filet.map > file.bed

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