I have a question about the Sure Select Multiplex kit - I notice that they use a different primer to that which is included in the Illumina multiplex sample prep kit. Does anyone know what the sequences of the Sure Select Indexing Pre-capture PCR primer and post-capture PCR primer are?
-
-
Its proprietary, I have asked. If anyone has it, I would be very interested in knowing it as well. -
I believe the pre-capture PCR primer is equivalent to the Illumina Multiplexing PCR Primer 2.0
The post-capture PCR primer is simply the Illumina Multiplexing PCR Primer 1.0 .
I cannot confirm this, but it makes logical sense.Comment
-
CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
Comment