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I am trying out the Burge lab's MISO program for isoform analysis. I want to use the mm9 file posted on their website, but within the mm9 folder are many subfolders with different pickled GFF annotations, I can't seem to get MISO to like any of these files. Which files do I feed MISO? Anyone else have this trouble, or have better experience using their own indexed alternative events file? Anyone gotten MISO to work?
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Yh, I have tried their hg18 GFF annotations for alternative splicing analysis, it works well. -
Miso
good to hear it is working for you - did you run it using the TandemUTR or A3SS/A5SS indexes? for the input file I navigate to the mm9_alt_events/home/yarden/sugarman/gff-events/mm9/pickled/TandemUTR folder -within that folder are the .shelve file and the individual chromosome folders containing pickle files. Trying to run from this folder gives me errors. What do I use as my input file? test_miso.py gives me an OK. Do I need to prepare the pickled files?
thanks, any help is appreciated.Comment
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I tried all the events including TandemUTR and A3SS/A5SS indexes, I think you can use their scripts and gff files to generate the pickled files ,not the final annotation files they already provided.Comment
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MISO - got it thanks
Thanks, I got it working now.Comment
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Miso vs. cufflinks
For miso users: What is the feature that distinguish miso from cufflinks, and helped you take miso as your favorite tool?Comment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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