I have a failed index read on a HiSeq run. I want to put new index read reagents on and go back to the beginning of the index read. However, my run has completed, and the instrument software won't let me choose where to resume the run. Does anyone know a way around this?
-
-
If it's a paired-end read, there's no way to salvage it after read two b/c the index primer anneals to the first strand. If it's a single-end read and you've already removed the flow cell, then you'll have to repeat the sequencing b/c the cluster positions won't align properly to the previous run. Use the rehybridization kit on the cBOT and go from there.
If it's single read and still on the machine, I think you'd need to remove all of the information (from log files, data folders, etc) for the index read, load the reagents, then restart that run. Unlike the GA, the recipe steps are not displayed during the run so it's more difficult to fiddle with the parameters.
Good luck,
Harold -
You can also email illumina's customer support at [email protected], they tend to be pretty good with their own systems, or call 800.809.4566.Originally posted by coleen_2 View PostComment
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Comment