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  • SeqClark
    Junior Member
    • Mar 2011
    • 2
    #1

    Create local BLAST database

    Hi there,

    I tried to use makeblastdb to create a local BLAST database and got the following error message. I checked the FASTA format of my file and it seemed to be OK. No idea how to correct this problem. Any help would be greatly appreciated.

    "Error: NCBI C++ Exception:
    "..\..\..\..\..\src\serial\objistrasnb.cpp", line 164: Error: ncbi::CObjectI
    StreamAsnBinary::UnexpectedTagClassByte() - byte 0: unexpected tag class/constru
    cted: #255, should be #160 ( at Seq-entry)"
    Tags: None
    • SeqClark
      Junior Member
      • Mar 2011
      • 2
      #2
      FAST sequence sample

      >gi|157676334|ref|NP_067082.2| transmembrane protein 8A precursor [Homo sapiens]
      MGRAGTGTGGEAVAAVVAGPLLLLLLARPPPASAGYSGKSEVGLVSEHFSQAPQRLSFYSWYGSARLFRF
      RVPPDAVLLRWLLQVSRESGAACTDAEITVHFRSGAPPVINPLGTSFPDDTAVQPSFQVGVPLSTTPRSN
      ASVNVSHPAPGDWFVAAHLPPSSQKIELKGLAPTCAYVFQPELLVTRVVEISIMEPDVPLPQTLLSHPSY
      LKVFVPDYTRELLLELRDCVSNGSLGCPVRLTVGPVTLPSNFQKVLTCTGAPWPCRLLLPSPPWDRWLQV
      TAESLVGPLGTVAFSAVAALTACRPRSVTIQPLLQSSQNQSFNASSGLLSPSPDHQDLGRSGRVDRSPFC
      LTNYPVTREDMDVVSVHFQPLDRVSVRVCSDTPSVMRLRLNTGMDSGGSLTISLRANKTEMRNETVVVAC
      VNAASPFLGFNTSLNCTTAFFQGYPLSLSAWSRRANLIIPYPETDNWYLSLQLMCPENAEDCEQAVVHVE
      TTLYLVPCLNDCGPYGQCLLLRRHSYLYASCSCKAGWRGWSCTDNSTAQTVAQQRAATLLLTLSNLMFLA
      PIAVSVRRFFLVEASVYAYTMFFSTFYHACDQPGEAVLCILSYDTLQYCDFLGSGAAIWVTILCMARLKT
      VLKYVLFLLGTLVIAMSLQLDRRGMWNMLGPCLFAFVIMASMWAYRCGHRRQCYPTSWQRWAFYLLPGVS
      MASVGIAIYTSMMTSDNYYYTHSIWHILLAGSAALLLPPPDQPAEPWACSQKFPCHYQICKNDREELYAV
      T

        Comment

        • rglover
          rg
          • Dec 2008
          • 51
          #3
          What version of the NCBI executables are you using? What command line arguments are you using to create your database?

            Comment

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