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Which programs suits aligning ABI solid reads most?
Which programs suits aligning 454 reads most?
Which programs suits aligning 454 reads most?
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If by "aligning" you mean "mapping to a reference sequence" then I would say that the company's offered software will be one of the best options. This is 'corona lite' for the Solid (works in color space!) and Newbler for 454.Originally posted by baohua100 View Post
For cross-platform mapping and/or assembly (i.e., mixing up 454 and SOLiD reads and expecting to obtain contigs from them) then neither of the above will work very well. -
Yes, i mean "mapping to a reference sequence" and i want to known some other programs designed for alignment of solid or 454 reads.Comment
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
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