Just curious!? At what point does the number of clusters max out the ability of the machine to read it well (from first base report) - on both a HiSeq or GA flowcell? Thanks
-
-
For the HiSeq, I have gotten great results when I aim for the 700,000 K/mm^2 cluster density off of the first base report (usually about 90-120 million reads pass filter). I have had some luck with shooting as high as 1,000,000 K/mm^2, but it's hit or miss and I wouldn't recommend it.
For the GAIIx, I usually aim for 350,000 clusters/mm^2 off of the first base report and about 35-40 million reads pass filter.
Hope this helps! -
About the GAIIx, I assume it is 350,000 clusters/tile?Originally posted by kthai View Post
Out of curiosity, what concentration are you loading on the GAIIx?
Best Regards.Comment
-
We typically load at 8pM, but this loading concentration may change depending on how multiplexed each lane is.
Also, it is important to note that this "8pM" loading concentration may vary for each lab depending on the choice of QC methods.Comment
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Comment