Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • JYC
    Junior Member
    • Jul 2008
    • 3

    Flow cell modifications ?

    Hello,
    Does anybody knows if there were some changes in the lengths and / or sequences of the primers attached to the Hiseq flow cells between version V.1 (ref : 15011379) and V1.5 (ref: 15020980) ?
    Thanks in advance for your support
  • cella
    Junior Member
    • Aug 2010
    • 3

    #2
    The lane width of version V.1 is 1.7mm, v1.5 is 1.8mm, but the valid width is the same.

    Comment

    • HESmith
      Senior Member
      • Oct 2009
      • 512

      #3
      It's my understanding that the manufacturing process was modified to improve clustering (more consistent primer coating, and elimination of edge effects) but that the primer sequences are unchanged.

      Comment

      Latest Articles

      Collapse

      • GATTACAT
        Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
        by GATTACAT
        Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
        07-01-2026, 11:43 AM
      • SEQadmin2
        Nine Things a Sample Prep Scientist Thinks About Before Sequencing
        by SEQadmin2


        I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

        Here are nine questions we think about, in roughly the order they matter, before...
        06-18-2026, 07:11 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, Today, 11:05 AM
      0 responses
      7 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 07-02-2026, 11:08 AM
      0 responses
      28 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-30-2026, 05:37 AM
      0 responses
      27 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-26-2026, 11:10 AM
      0 responses
      26 views
      0 reactions
      Last Post SEQadmin2  
      Working...