Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • ashwatha
    Member
    • Jul 2011
    • 14

    Samtools faidx errors

    Hi,

    I am new to samtools and bioinformatics in general. I have a quick question about generating a fasta index file from a .fa file. I see a segmentation fault while running samtools faidx on hg19:

    $samtools faidx hg19.fa
    [fai_build_core] different line length in sequence 'chr1'.
    Segmentation fault

    This is probably a dumb question, but what could be causing this?

    Thanks!
  • nilshomer
    Nils Homer
    • Nov 2008
    • 1283

    #2
    You have differing line lengths after chromosome one. Check that each line in chr1 has the same length (except for the last, which should be equal to or shorter).

    Comment

    • ashwatha
      Member
      • Jul 2011
      • 14

      #3
      Thanks - I didn't know that every line had to be the same length.

      Comment

      • annrose
        Junior Member
        • Jun 2014
        • 4

        #4
        samtools faidx hg19.fa
        [fai_build_core] different line length in sequence 'chrY'

        hi i have the same problem.....can you tell how can i correct the length?? i am very new to these stuff

        Comment

        Latest Articles

        Collapse

        • SEQadmin2
          Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems
          by SEQadmin2



          Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
          ...
          07-09-2026, 11:10 AM
        • SEQadmin2
          Cancer Drug Resistance: The Lingering Barrier to Rising Survival
          by SEQadmin2



          Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.

          There is no single reason why many patients don’t respond to treatment as expected. Cancer is...
          07-08-2026, 05:17 AM
        • GATTACAT
          Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
          by GATTACAT
          Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
          07-01-2026, 11:43 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by SEQadmin2, 07-13-2026, 10:26 AM
        0 responses
        27 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 07-09-2026, 10:04 AM
        0 responses
        37 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 07-08-2026, 10:08 AM
        0 responses
        24 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 07-07-2026, 11:05 AM
        0 responses
        34 views
        0 reactions
        Last Post SEQadmin2  
        Working...