Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • jordi
    Member
    • Apr 2009
    • 49

    #1

    what's clipping point?

    Hi!
    I'm working with de novo 454 data and , after a GS FLX asemmbly, I try to see the results with clview which works with .ace files and I can see something like the attachment file. The reads have right and left clip. What does it mean? I found anything about this clipping point. Can I change some value in order to improve the assembly?
    Thank you very much!
    Attached Files
  • flxlex
    Moderator
    • Nov 2008
    • 412

    #2
    Reads are quality trimmed before assembly, the new start and stop are the left and right clipping points. Usually, the left (5') side is never trimmed (beyond the first four bases that are common for all reads).

    Trim values can be changed using the sfffile command, check the manual.

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM
    • SEQadmin2
      New Genomics Technologies Take Aim at Long-Standing Limits
      by SEQadmin2


      Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

      We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
      ...
      09-28-2026, 10:25 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 10-22-2026, 11:13 AM
    0 responses
    31 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 10:48 AM
    0 responses
    23 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-15-2026, 10:52 AM
    0 responses
    40 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-14-2026, 05:39 AM
    0 responses
    45 views
    0 reactions
    Last Post SEQadmin2  
    Working...