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  • leesi
    Junior Member
    • Jul 2011
    • 1
    #1

    Experience of Illumina TruSeq Exome coverage?

    Hi,
    I'm wondering what coverage others are seeing with the Illumina TruSeq Exome, specifically % bases with either 10, 15 or 20x coverage. We're running 6 samples in a pool, across 2 lanes - any idea what we should expect? An average measure isn't as helpful, since I'm more keen to quantify what we're missing.
    Cheers!
    Tags: None
  • NextGenSeq
    Senior Member
    • Apr 2009
    • 482
    #2
    Roughly 2/3rds are greater than 10X coverage when a single exome is run in a HiSeq lane. This wasn't a v3 run though.

    Comment

    • chongm
      Member
      • Sep 2012
      • 21
      #3
      Truseq 62 Mb exome enrichment

      With the 62 Mb exome enrichment, on a hiseq lane we 6-plexed and got ~70%PCTbases > 20x

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