Tweet
Visualization of variants from varScan with IGV
Hi all,
I visualized the variant positions I got from varScan using IGV.
I saw that they show different coverage from what I have detected using varScan (usually the IGV have higher coverage for both variant and reference).
This was also the case when I only used filtering for variant base quality>=15 and the other filtering steps done by awk on the output files.
Example:
from varScan output:
chr1 556810 T Y 4625 254 5.21% 2 2 30 30 0.98 1 1 2266 2359 3 251 C
from IGV:
chr1 556810:
A: 169 (2%,0+,169-)
C: 692 (8%, 5+, 687-)
T: 7551 (90%, 4316+, 3225-)
N: 0
Does anyone know where I got wrong?
Thanks in advance,
Oz
I visualized the variant positions I got from varScan using IGV.
I saw that they show different coverage from what I have detected using varScan (usually the IGV have higher coverage for both variant and reference).
This was also the case when I only used filtering for variant base quality>=15 and the other filtering steps done by awk on the output files.
Example:
from varScan output:
chr1 556810 T Y 4625 254 5.21% 2 2 30 30 0.98 1 1 2266 2359 3 251 C
from IGV:
chr1 556810:
A: 169 (2%,0+,169-)
C: 692 (8%, 5+, 687-)
T: 7551 (90%, 4316+, 3225-)
N: 0
Does anyone know where I got wrong?
Thanks in advance,
Oz
Comment