Can Truseq DNA library get >1ug DNA library at last?

rnal

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Join Date: Jul 2011

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Can Truseq DNA library get >1ug DNA library at last?

08-16-2011, 02:28 AM

We make the DNA library with the Truseq DNA library protocol :end repair, A-Tailing, ligase, PCR enrich (11 cycles )。
input DNA： 2 ug 250-400bp DNA
Can get >1ug library DNA at last?

now we want to make the Exome Library with NimblelGenSeqCapEZ, NimblelGen require the library(illumina Truseq DNA protocol,11cycles PCR) before capture is 1 ug. And we do severl times ,find that it cannot get 1ug DNA after 11 cycles PCR. (NimblelGen LM-PCR1 is 8 cycles)

is somebody use the NimblelGen Exome capture library?

thank you!
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fluo

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08-25-2011, 06:25 PM

me too.
Does anybody know the solution?
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zwirko

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Join Date: Feb 2011

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04-12-2012, 07:36 AM

Use Kapa HiFi, you should have plenty of material
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