My facility has recently purchased an Illumina GA IIx. I was wondering for qPCR during sample prep, what kits/reagents people recommend? We have an ABI stepOnePlus instrument.
Thanks!
Thanks!
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KAPA kit I would recommend -
If you're looking for a SYBR (or similar)-based master mix, I can tell you that Fisher Scientific has a promotional deal on Promega's GoTaq qPCR mix that is ridiculously cheap. It is sold as 1000 or 200 reactions, but that's based on a 50uL reaction volume, so if you do 25uL reactions, that's the price you'd get for 2000 or 400 reactions.
GoTaq qPCR promoComment
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