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  • gavin.oliver
    Senior Member
    • Jan 2010
    • 110
    #1

    Coverage required for Sanger based SNP detection and Genotyping

    Does anyone know of an established acceptable figure in terms of times coverage required for SNP/calling and genotyping with Sanger sequences?

    I know 20x is generally recommended for NGS but this is obviously a different scenario.
    Tags: None
  • sciencewu
    Member
    • Dec 2010
    • 12
    #2
    many papers using the Sanger sequences ,but didn't give a criteria . maybe 10x is enough.

    Comment

    • prm36
      Member
      • Mar 2010
      • 16
      #3
      We take 2x coverage for SNP calling in our lab.

      Ventner's genome was done to 7.5x (http://www.nature.com/nrg/journal/v1...df/nrg2626.pdf)

      Cheers

      Paul

      Comment

      • sciencewu
        Member
        • Dec 2010
        • 12
        #4
        Did you get a good result ? what's FP and FN ?

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