Illumina TruSeq resuspension Buffer

[lvandiepen]

Hello,
Does anyone know what the resuspension buffer in the TruSeq RNA library prep kit contains/what the ingredients are? Is it comparable with 10 mM TRIS?

Thanks,
Linda

[NextGenSeq]

I'm sure it also contains Tween-20. They recommend adding it to prevent the DNA sticking to the tube

[marca]

10 mM Tris-HCl, pH 8.5

[MAdkisson]

Is anyone certain about whether or not Tween-20 is involved?

[NextGenSeq]

We resuspend all our libraries in Low TE with 0.1% Tween-20

The Tween-20 prevents libraries sticking to the microtubes.

[linvei91]

Is it ok to submit for sequencing in those resuspension buffer of Truseq RNA library kit?

[jdk787]

Is it ok to submit for sequencing in those resuspension buffer of Truseq RNA library kit?

Yes it is.