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  • Geneious
    Registered Vendor
    • Jul 2010
    • 22

    Assemble RNA-Seq data with TopHat in Geneious Server!

    Geneious Server™ 1.5 Beta, the high performance enterprise extension of Geneious Pro now features TopHat for mapping millions of RNA-Seq data against a reference genome in minutes. Click on the Assembly Button, choose "Run on Geneious Server" and select TopHat as your algorithm of choice from the dropdown menu of assemblers & mappers.

    Learn More | Enquire Now

    "Geneious has done a great deal for our lab, and I think it adds incredible value for labs with a mixture of wet and dry folks or those doing lots of cloning like ours. TopHat is a robust tool, but it was designed with experienced bioinformatics scientists in mind. Geneious makes TopHat accessible to many more users through the same powerful, intuitive user interface they have with other Geneious analysis tasks."
    Dr. Cole Trapnell, Author of TopHat and Post-Doc in the Rinn Lab at Harvard University


    For further details, read on here

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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
    07-01-2026, 11:43 AM
  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM

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