Unconfigured Ad

Collapse
X
Collapse
 
  • Page of 1
  • Filter
  • Time
  • Show
Clear All
new posts
Previous template Next
  • fadista
    Member
    • Sep 2008
    • 37
    #1

    multiplex few variants in hundreds of samples

    Dear all,

    I would like to know if someone is working (or knows someone that is working) on a method to be able to multiplex sequencing of very few variants (let say 100 SNPs) on hundreds or maybe thousands of samples in a single HiSeq run. There is a market need for this kind of product but as far as I know, no solutions are yet available.
    Tags: hiseq, multiplexing
  • Heisman
    Senior Member
    • Dec 2010
    • 534
    #2
    Would this be appropriate? http://www.illumina.com/technology/g...ing_assay.ilmn

    Comment

    • Bukowski
      Senior Member
      • Jan 2010
      • 388
      #3
      Originally posted by fadista View Post
      Dear all,

      I would like to know if someone is working (or knows someone that is working) on a method to be able to multiplex sequencing of very few variants (let say 100 SNPs) on hundreds or maybe thousands of samples in a single HiSeq run. There is a market need for this kind of product but as far as I know, no solutions are yet available.
      Already answered elsewhere on the forums:

      Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex - PubMed
      http://www.ncbi.nlm.nih.gov/pubmed/18264105
      We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of s …

      Comment

      Previous template Next

      Latest Articles

      Collapse
      • SEQadmin2
        From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data
        by SEQadmin2


        Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.

        The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
        ...
        06-02-2026, 10:05 AM
      • SEQadmin2
        Single-Cell Sequencing at an Inflection Point: Early Impacts of New Platforms and Emerging Trends
        by SEQadmin2


        With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.

        Introduction

        Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...
        05-22-2026, 06:42 AM
      • SEQadmin2
        Environmental Genomics in the Age of NGS: From Microbes to Conservation Strategies
        by SEQadmin2

        Studying ecosystems means dealing with complex, multi-species communities that are hard to observe at scale. This complexity, however, hides many important questions to be answered, from how biogeochemical cycles work and how climate change can affect species distribution to how conservation strategies can work best.

        Genomics, particularly since the expansion of NGS, has transformed ecosystem ecology. By sequencing environmental DNA, we can now assess biodiversity without direct...
        05-06-2026, 09:04 AM
      View All

      ad_right_rmr

      Collapse

      News

      Collapse
      Topics Statistics Last Post
      A New Single-Cell Method Maps DNA-Protein Interactions by SEQadmin2
      Started by SEQadmin2, Today, 08:59 AM
      0 responses
      11 views
      0 reactions
      		 Last Post SEQadmin2
      by SEQadmin2
      Today, 08:59 AM  
      Long-Read RNA Sequencing Uncovers a Hidden Layer of Immune Cell Regulation by SEQadmin2
      Started by SEQadmin2, 06-02-2026, 12:03 PM
      0 responses
      21 views
      0 reactions
      		 Last Post SEQadmin2
      by SEQadmin2
      06-02-2026, 12:03 PM  
      DNA Methylation Study Reveals How Epigenetic Changes Pass Between Generations by SEQadmin2
      Started by SEQadmin2, 06-02-2026, 11:40 AM
      0 responses
      17 views
      0 reactions
      		 Last Post SEQadmin2
      by SEQadmin2
      06-02-2026, 11:40 AM  
      MetaBeeAI Helps Scientists Process Research Literature Faster by SEQadmin2
      Started by SEQadmin2, 05-28-2026, 11:40 AM
      0 responses
      31 views
      0 reactions
      		 Last Post SEQadmin2
      by SEQadmin2
      05-28-2026, 11:40 AM  
      View All
      Working...