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So I can't find the formulation/concentration of the Low TE that comes with the Ion kits anyone know what the concentration and pH of the Tris-EDTA should be?
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Low TE generally is 10 mM Tris pH 8 0.1 mM EDTA, don't know for sure if Ion Torrent uses that. -
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Hi there
Is this Low TE then acceptable to use for diluting gDNA in the Ion Shear fragment Xpress kit? I did an ethanol precipitation and re suspended the gDNA in RNase-free dH2O to be safe but I would prefer to use it as is, if it wont affect the reaction.
JuliJuli Hepple (MSc Genetics)Central analytical facilitiesDNA Sequencing UnitSouth AfricaComment
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Yes, I would use this buffer to resuspend your DNAs.Comment
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