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Does anyone know the current cost per run for the GAII, in terms of consumables etc.? How much does it change if you're just doing one lane? Any help would be awesome.
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Hi,
Here are my numbers which I give absolutely no guarantees on! They are a pretty good indication of the consumables needed per lane including flow cell and sequencing reagents. All costs assume one lane is used for PhiX and the other lanes share that cost. The numbers in brackets are what we currently exepct to get from a 36bp kit. We will wait and see what formats the new kits will come in, here's hoping for a 150bp sequencing kit!
I hope you are all as stunned as I was when I saw that a 2x150bp lane costs only £1450 (£10K per flow cell). This brings the possibility of nearly 20M 250bp contigs using a 2x150bp read from a 250bp insert library. What can you NOT do with that?
1 lane of SE sequencing without library prep 36bp (44) £ 375
1 lane of SE sequencing without library prep 72bp (88) £ 500
1 lane of SE sequencing without library prep 108bp (132) £ 650
1 lane of SE sequencing without library prep 144bp (176) £ 750
1 lane of PE sequencing without library prep 36bp (44) £ 700
1 lane of PE sequencing without library prep 72bp (88) £ 950
1 lane of PE sequencing without library prep 108bp (132) £ 1200
1 lane of PE sequencing without library prep 144bp (176) £ 1450 -
Thanks for the info, James. Greatly appreciated.
Do people know if James' info is in line with what users end up paying in the US? Do they quote out lower prices for volume users?Comment
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What is the current cost of fragment library preps and number of sequences per lane on good runs?Comment
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up to 18M reads on GA2, should be almost 20M on a good day from GA2x pipeline 1.4.
Library preps are about £250 per sample from Illumina but the oligo only kit can reduce this substantially. It is very dependant on which kit you want to use.Comment
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We cost about USD 1400 dollars per lane with 75bpXII PE sequencing per lane.Comment
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I think the core facility I've been using charges us about $450 per lane. I was told it will increase though, perhaps about 10%.Comment
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
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