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Does anyone have experience using Illumina HiSeq v3 reagents after their expiry date? Everything is still stored at -20 in original packaging. Kits in question is SR and PE v3 clustering kits and sequencing kits. If so, how far out have you used them? Be aware, my Illumina products arrived with only 3 months left on the shelf life!
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As long as it was stored properly, I don't see what could have gone bad.... -
I was told by my FAS that the SR kits are more robust to being used past expiry than the PE kits. I guess it has something to do with how the linearisation is achieved.
We have some SR kits that are about 6 months out of date and we're going to put them on in a few weeks to see if we get anything, but I have no info at the moment.Comment
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Highly curious: Did you ever run any of the expired SR cluster kits?Comment
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
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