I started working on whole genome sequencing and exome sequencing with Illumina HiSeq 2000 and also with 454 sequencer. Can anybody please help me to understand the data analysis of the sequenced reads????
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If you'd like answers to such a question, you should start by posting in the correct forum. Then, explain what you want to do with the reads. You're query can be condensed to "I ran an experiment; how do I analyze it?", and nobody can really help you without some real detail.Originally posted by rd69 View Post -
Anyone remember the days were you just dumped 3 volumes of Maniatis on a student desk and turned around... probably not.Originally posted by rd69 View PostComment
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Moved this to a new thread...and I really encourage the original poster to read the following:
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Yesterday, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
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