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We are looking at a small pilot project, 50-100 exomes (we prefer Illumina based core). Already have some exp with OtoGenetics but their SureSelect capture based option seems pricey. Anyone ? Thanks (not sure if that is the right category to ask for it but....)
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Last edited by memento; 02-15-2012, 09:54 AM. -
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We've tested a number of exome service providers and are now going to regularly use Axeq.
We were quoted <£700 per exome using Nimblegen's latest capture.
We had a number of communication problems and delays with BGI.Comment
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What was the matter...wasn't Ryan getting back to you in time?Originally posted by TonyBrooks View Post
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$999 exome
We do $999 at the Columbia Genome Center. That includes all reagents, Agilent SureSelect XT Human All Exon v.2 44Mb, Library preparation, 60X depth mapped coverage, 100bp Paired End reads, sequenced on Illumina HiSeq2000 instruments, 2 months data storage, QC, reads mapped to the human reference genome, single nucleotide variants and short insertions/deletions, basic functional annotation of the variants, and summary statistics. Our average turnaround was 55 days last quarter.
I can send you recommendations from PIs who use our service. We take orders from all over the world.
We will be happy to answer any questions at [email protected]
Our FAQ is here:
Last edited by ocouronne; 02-21-2012, 10:58 AM.Comment
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so can you detail the problems?maybe we can give you feedback.Originally posted by TonyBrooks View PostComment
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If he is in the UK, shouldn't your European subsidiary be addressing these issues? Or do they lack the competence to do so?Originally posted by Gone View Post
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Yeah,U're right!BGI-Europe will handle all issues in local region.Originally posted by Geneus View PostIf he is in the UK, shouldn't your European subsidiary be addressing these issues? Or do they lack the competence to do so?
Anyway,we'll try our best to do any kinds of problems.
you know customer forever is God!
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Yes...that's the way Mr. Mason would want it.Originally posted by Gone View PostYeah,U're right!BGI-Europe will handle all issues in local region.
Anyway,we'll try our best to do any kinds of problems.
you know customer forever is God!
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Best and smartest sequencing facility around is Expression analysisComment
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This should (perhaps obviously) be noted as biased.Originally posted by kamichailo View PostComment
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
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