Integrating MiSeq in an existing HiSeq infrastructure

[GenoMax]

This question is for people who may have both HiSeq and MiSeq instruments.

Based on what I have read here in the past, MiSeq strives to be a stand-alone instrument with most of the data capture and analysis happening on the instrument computer.

I have following questions:

1. Is is possible to turn the default analysis off and just write the data (either locally or to a samba share mounted on the MiSeq computer) for off-line analysis? We already have an infrastructure in place (with several HiSeq's in house) for downstream analysis that we would like to use.

2. If #1 is not easily feasible then how are you handling the data analysis/integration with other workflows (for HiSeq machines).

3. I hope the cloud based data analysis (BaseSpace) is optional and can be turned off completely.

Thanks in advance.

[hoisinjl]

1. We have a samba share drive mounted on the MiSeq that the data is transferred to during the run. We also just have fastq files generated and all the data is run through our own off-line analysiss.
3. BaseSpace is optional. Just need to unselect the option at the beginning of the run.

[GenoMax]

Thanks for that info.

As an aside: Do you have your instrument computer behind a firewall? If not, are you running any anti-virus software on the MiSeq computer?

1. We have a samba share drive mounted on the MiSeq that the data is transferred to during the run. We also just have fastq files generated and all the data is run through our own off-line analysiss.

[behavin]

From experience with HiSeq's, I'd say leave off on installing anti-virus software, or anything else that you'd don't have to have really. All of our instrument workstations are behind firewalls to avoid tinkering with anti-virus.

[GW_OK]

Don't install anything that might take any CPU time or RAM or that would want to sit there and scan files, those computers are already taxed as it is!