Maximum Insert Size of Illumina Libraries?

[andyding]

Hi, all:
I'm wondering what is the maximum insert size of illumina paired-end or mated-paired libraries? Some source saids it's 20kb or 40kb, I'm wondering if it is possible that I can get a even longer insert size?
Moreover, will library preparing process become more complicated or harder when the insert size increases?
Thanks, and all the best.

[SeqAA]

If you are talking about mate pair, there are people that can go up to 40kb+. This is not something the average user will be able to accomplish.

[andyding]

Thanks for your reply.
I was wondering if you can tell me what is the potential technical difficulties when constructing libraries with ultra-large insert size?
Thanks again.

Andy

If you are talking about mate pair, there are people that can go up to 40kb+. This is not something the average user will be able to accomplish.

[hawaii454-0]

Hi,
Does anyone have final libraries which show shoulder peaks on their larger insert libraries?

Thanks,
Anna.