[Robby]

Hello everyone,

I have a look for the cluster densities for the first time. Do you use for the cluster density the number out of the first base report or the cBot number or any other source? I am just asking because our number of clusters vary between the cBot and the first base report.

Thanks
Robby

[pmiguel]

Hello everyone,

I have a look for the cluster densities for the first time. Do you use for the cluster density the number out of the first base report or the cBot number or any other source? I am just asking because our number of clusters vary between the cBot and the first base report.

Thanks
Robby

Unless I am missing something, the cBot has no capability to assay cluster density.

On the instrument there is a very inaccurate attempt at cluster density determination made after the first cycle. This is included in the first base report, or "FBR". Then after the 4th cycle another determination is made and for all intents and purposes that is the cluster density.

However, the cluster density, as measured by the instrument software is not always accurate. At very low, or very high (>1000 Kclusters/mm2) densities the cluster density will be substantially below what the "real" density is.

None of the above considers the next layer of processing that determines the "PF" or Pass Filter clusters.

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Phillip

[Robby]

Thanks Philip. You wrote, that after the 4th cycle another cluster density determination is made. Is that cluster density the same as written in the final run statistics? Or where can we find the cluster densities after the 4th cycle?

[Robby]

OK, I found the answer: The cluster density will not change after cycle 4, so the final cluster density is the same like the 4th cycle cluster density.

[pmiguel]

OK, I found the answer: The cluster density will not change after cycle 4, so the final cluster density is the same like the 4th cycle cluster density.

Yes, that is correct.

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Phillip