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Old 08-01-2009, 02:16 PM   #8
wiggy
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Location: Lexington KY

Join Date: Jun 2009
Posts: 4
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We subjected RNA to 2 rounds of PolyA purification from Promega and also to 1 round of Ribominus.

After analyzing via qRTPCR for both 18S and 28S rRNA we found the PolyA kit almost completely eliminated rRNA after 2 rounds.

Next we created primers for some large genes near the extreme 5' region. Some of these had been previously verified as low expression levels via more 3' primers. We found these transcripts to be represented in the polyA 2x.

Ribominus was not effective at all, perhaps because our rRNA's did not contain the consensus sequence.
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