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  • Gorbenzer
    Member
    • Apr 2012
    • 45
    #1

    Alternative (cheaper) reagents for Ion Torrent PGM sequencer

    Hi everyone,
    in my lab we are trying to find a way for reduce the running costs of our new IonTorrent PGM sequencer... any suggestion?

    Anyone found cheaper reagents that can substitute the ones sold by Life Technology?
    Tags: None
  • jimmyinfrance
    Member
    • May 2011
    • 16
    #2
    We have checked out NEB kits, work OK (although still more expensive than I'd like).

    Comment

    • Gorbenzer
      Member
      • Apr 2012
      • 45
      #3
      Originally posted by genseq
      Cheap barcoding:
      Looks really interesting, time to use google translator
      Last edited by Gorbenzer; 06-05-2012, 11:29 PM.

      Comment

      • diagen
        Member
        • Jun 2012
        • 13
        #4
        Does anybody know the sequences of primers, adaptors and immobilized oligos?

        Comment

        • HeidiJTP
          Member
          • Nov 2011
          • 22
          #5
          genseq - thanks for the latest info!

          How about the NEXTflex kits? Anyone had experience with their DNA-Seq kits or the barcoding?
          http://www.biooscientific.com/DetailProduct.aspx?itemId=459&CName=Next-Generation%20Sequencing&SubName=Ion%20Torrent-Compatible&Name=DNA%20Sequencing

          Comment

          • prooney
            Junior Member
            • Oct 2012
            • 2
            #6
            Has anyone tried halving the AmpliSeq PCR reactions? I understand this would change the PCR dynamics...

            Comment

            • salyamoff
              Junior Member
              • Sep 2013
              • 4
              #7
              Originally posted by prooney View Post
              Has anyone tried halving the AmpliSeq PCR reactions? I understand this would change the PCR dynamics...
              This is intresting question. Nobody ever tried?

              I did it with the cancer hotspot panel. Sequencing results are similar.
              Last edited by salyamoff; 04-03-2014, 11:23 AM.

              Comment

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