Alternative (cheaper) reagents for Ion Torrent PGM sequencer

[Gorbenzer]

Hi everyone,
in my lab we are trying to find a way for reduce the running costs of our new IonTorrent PGM sequencer... any suggestion?

Anyone found cheaper reagents that can substitute the ones sold by Life Technology?

[jimmyinfrance]

We have checked out NEB kits, work OK (although still more expensive than I'd like).

[Gorbenzer]

Cheap barcoding:

Looks really interesting, time to use google translator

[diagen]

Does anybody know the sequences of primers, adaptors and immobilized oligos?

[HeidiJTP]

genseq - thanks for the latest info!

How about the NEXTflex kits? Anyone had experience with their DNA-Seq kits or the barcoding?

http://www.biooscientific.com/DetailProduct.aspx?itemId=459&CName=Next-Generation%20Sequencing&SubName=Ion%20Torrent-Compatible&Name=DNA%20Sequencing

[prooney]

Has anyone tried halving the AmpliSeq PCR reactions? I understand this would change the PCR dynamics...

[salyamoff]

Has anyone tried halving the AmpliSeq PCR reactions? I understand this would change the PCR dynamics...

This is intresting question. Nobody ever tried?

I did it with the cancer hotspot panel. Sequencing results are similar.