So you did a test run and it appeared to work, then you did a regular run and all your beads went to waste again....is that correct?
-
-
-
I think this goes back to what "snetmcom" said, if there are beads in the waste then they must have passed through the filter at some stage. They shouldn't be able to do this so it must be the source of your problem.
There are several washes of your beads on the filter, during one wash an aliqout is taken off to the QC output (before enrichment). This should happen even if your emPCR has failed. If you're not getting anything in the QC output then it looks like your beads are passing through the filter for some reason. Probably a good idea to check your lot numbers with your rep.
JPCComment
-
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
Comment