RNAseq by PacBio

[Ddhamburger]

Hi, all
I was wondering if PacBio is an idea platform for RNAseq due to its long read length.
Or Is it good to combine PacBio data with illumina's?
Thanks

[ECO]

For looking at isoforms of a small set of genes, almost definitely, pending you can make ds cDNA long enough.

Almost certainly not enough tput to be useful for whole transcriptome.

[GenoMax]

PacBio is also going to be more expensive overall. Error rate with PacBio will cause problems unless you have other supplemental data (illumina, Solid etc) or a good reference sequence.