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  • ETHANol
    Senior Member
    • Feb 2010
    • 308
    #1

    ChIP - shearing with the Covaris S2

    I've been doing some optimization work with ChIP and the Covaris S2. I figure some people would probably be interested. All in all it appears to be a great instrument. Fragmentation of the chromatin is really even without bias towards open chromatin which is important for ChIP-seq. My only complaint is that it only takes one sample at a time, so if you have a lot of samples to process it can take a good amount of time. But the reproducibility is worth the extra time.

    Anyway, it's too much to post here but it's on my blog at:
    Covaris, Covaris, Covaris
    http://ethanomics.wordpress.com/2011/11/30/177/
    Like just about everything my Covaris ChIP protocol seems to work much better for some epitopes than others. My guess is that this primarily has to do with the degree of crosslinking. So I decided …

    X-linking, Shearing and ChIP with the Covaris S2
    http://ethanomics.wordpress.com/2011/12/28/202/
    I decided to look at how crosslinking conditions affect ChIP for a histone variant I am working on. I used three crosslinking conditions and shearing times which would shear the chromatin well. Eac…

    ChIP (fragmentation with Covaris) Protocol
    http://ethanomics.wordpress.com/chip-fragmentation-with-covaris-protocol/
    ChIP (fragmentation with Covaris) Protocol


    Thanks to Hamid at Covaris for his help. Phenomenal customer support.
    --------------
    Ethan
    Tags: None
  • Liting
    Member
    • Jul 2011
    • 12
    #2
    Why I can't open the Links?
    Can you give me the new links?Thanks !

    Comment

    • ETHANol
      Senior Member
      • Feb 2010
      • 308
      #3
      The links seem to be fine. Are you still having this problem? Send me a PM if you like.
      --------------
      Ethan

      Comment

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