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Originally posted by GenoMax
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IMHO this is not necessary; Lane X should look good, that's more important :-) We do this from time to time with chipseq data. -
If there was a control lane defined then there should be no need to go messing about with matrix or phasing settings (assuming the control lane looked OK).Originally posted by victorsor View Post
If you've still got no clusters then the only remaining remedy would be to run the bclToFastq with the --no-eamss option to see if the qualities temporarily dip and then recover.Comment
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Thank you all. We will try to do your suggestions when run finish. I hope that not all data go to waste...Comment
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
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