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  • litc
    Member
    • Oct 2010
    • 24

    #1

    Are RAD data strand-specific?

    I think RAD data are not strand-specific, we can sequence both the two strand of DNA, but I found ustacks do regard one sequence and it's reverse complementary strand as different loci. Do anyone know are RAD strand-specific or not?
  • DZhang
    Senior Member
    • Jun 2010
    • 177

    #2
    Litc,

    RAD is strand-specific; it is determined when the RAD adapter is ligated.

    Best regards,
    Douglas

    Comment

    • litc
      Member
      • Oct 2010
      • 24

      #3
      Originally posted by DZhang View Post
      Litc,

      RAD is strand-specific; it is determined when the RAD adapter is ligated.

      Best regards,
      Douglas
      www.contigexpress.com
      Thank DZhang, I see it.

      Comment

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