Bioanalyzer expired RNA Nano 6000 kit

[arkal]

Hey!

I'm need to run a preliminary QC on my RNA samples but I've got a problem... My RNA Nano 6000 kit expired in june 2010 (yes yes lmao ).

I wanted to know whether only reagents have an expiry date or does the chip also have an expiry date? I have 90+ unused chips all from the same lot (expiring June 2010) and dont know whether i can run my assay on the same chips if i buy fresh reagents and ladder or whether i need to buy new chips as well. Any help will be appreciated!

thanks in advance
Arkal

[cascoamarillo]

Hi,
I've used expired RNA pico reagents and chips (>1 year) without to much trouble (from different lots); it gave me really good profiles. Maybe, the ladder is the thing that shouldn't be too old. I think your chips from 2010 are gonna be fine with fresh reagents. Let me know how it works if you do so.
Cheers

[pbluescript]

I just wanted to second cascoamarillo's comments. I've never had a problem with old chips. The dye and ladder are the reagents that have given me trouble if they are old.

[arunkh]

Hi,

You shouldnt be having any problem with the Chips whatsoever. But the reagent should be gone. The reagents have to be reordered. RNA 6000 reagents were a lot of trouble to us even when it hadn't expired.