[Pila]

Hi everybody,
We are using Dynabeads which are not blocked by any carrier DNA so I also think the reason may be due to something else...what I can tell you is that even if you get this strange pattern it does not mean that the sequencing will be bad...

[Pila]

Actually,
Could it be that what we see corresponds to ssDNA enriched by the IP...since I just red on this forum that ssDNA is running very slowly compare to dsDNA on the DNA chip?

[pmiguel]

Post a picture of your gel/bioanalyzer chip. Yes, ssDNA can run larger than dsDNA on a bioanalyzer chip.

--
Phillip

[MGineste]

I reproducibly observed the same pattern. I may have an explanation for this, that I already posted there : http://seqanswers.com/forums/showthr...50&postcount=3

[Shubhamoy]

Can somebody please tell me what should be the ideal size of frangments after IP if samples are run at bioanalyser/ Agarose gel?