View Single Post
Old 09-24-2009, 11:56 PM   #10
Junior Member
Location: South Africa

Join Date: Nov 2008
Posts: 3


Our latest batch of libraries looks much better, with library preps only using the default Illumina mRNA-Seq kits (and whatever beads they contain). rRNA content seemed to be only around 2%-4% for each of the six libraries. Seems the problem was only in the old sample prep method before the dedicated RNA-Seq kits came out, and 2 rounds of mRNA selection using magnetic beads did the trick. Can't comment on Ribominus then, at the moment we're still only looking in poly-adenylated RNA.

Thanks again for the advice.
Eshchar is offline   Reply With Quote