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Old 09-24-2009, 11:56 PM   #10
Eshchar
Junior Member
 
Location: South Africa

Join Date: Nov 2008
Posts: 3
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Hi,

Our latest batch of libraries looks much better, with library preps only using the default Illumina mRNA-Seq kits (and whatever beads they contain). rRNA content seemed to be only around 2%-4% for each of the six libraries. Seems the problem was only in the old sample prep method before the dedicated RNA-Seq kits came out, and 2 rounds of mRNA selection using magnetic beads did the trick. Can't comment on Ribominus then, at the moment we're still only looking in poly-adenylated RNA.

Thanks again for the advice.
Eshchar
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