The last run was on a v2 MiSeq with v1 chemistry. 1,000,000 usable reads at >q20, which if clustering your sequences at 97% (to account for sequencing errros) should be accurate enough for most things. Next run will be v2. I'll post the metrics for that run in a week or two.
-
CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
Comment