Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • upper
    Member
    • May 2010
    • 10

    #1

    picard SamToFastq error in umapped reads which MAPQ is not 0

    Hi,
    SamToFastq.jar will be stop when it meet the unmapped reads which flag is 4 or 20 but the MAPQ is not 0. Picard is not so smart,it couldnot skip this reads but stop to deal next reads.
    I am also confused why unmapped reads MAPQ is not 0? I found many of unmapped reads which flag is 4 or 20 has MAPQ and OPT column. MAPQ 37 is the highest sore in bwa reasult and normal means its unique mapping reads.
    I use bwa Version: 0.6.1-r104 to do mapping.
    Can someone know how to deal these reads,I want to mapping unmaped reads to junction?


    Code:
    java -jar SamToFastq.jar INPUT=s_3_2_unmap_genome.sam FASTQ=s_3_2_unmap_genome.fq
    
    
    Wed Dec 05 09:27:59 HKT 2012] net.sf.picard.sam.SamToFastq done. Elapsed time: 1.19 minutes.
    Runtime.totalMemory()=262864896
    FAQ:  http://sourceforge.net/apps/mediawiki/picard/index.php?title=Main_Page
    Exception in thread "main" net.sf.samtools.SAMFormatException: Error parsing text SAM file. MAPQ should be 0 for unmapped read.; Line 3205583
    Line: HWUSI-EAS724_0039:3:34:15714:3092#0       20      9       124595110       37      80M     *       0       0       AGTTAATGTAGCTTAATAACAAAGCAAAGCACTGAAA
    ATGCTTAGATGGATAATTGTATCCCATAAACACAAAGGTTTGG        hggh]hghhhghghhhhhghhhhhhhhhhhdhhhhhhhcfhhghhhhhhhchhhghhfdhhhghhhhhhgghhghhghhh        XT:A:U  NM:i:1  XN
    :i:1  X0:i:1  X1:i:0  XM:i:1  XO:i:0  XG:i:0  MD:Z:0G79
            at net.sf.samtools.SAMTextReader.reportErrorParsingLine(SAMTextReader.java:230)
            at net.sf.samtools.SAMTextReader.access$500(SAMTextReader.java:40)
            at net.sf.samtools.SAMTextReader$RecordIterator.parseLine(SAMTextReader.java:434)
            at net.sf.samtools.SAMTextReader$RecordIterator.next(SAMTextReader.java:278)
            at net.sf.samtools.SAMTextReader$RecordIterator.next(SAMTextReader.java:250)
            at net.sf.samtools.SAMFileReader$AssertableIterator.next(SAMFileReader.java:641)
            at net.sf.samtools.SAMFileReader$AssertableIterator.next(SAMFileReader.java:619)
            at net.sf.picard.sam.SamToFastq.doWork(SamToFastq.java:129)
            at net.sf.picard.cmdline.CommandLineProgram.instanceMain(CommandLineProgram.java:177)
            at net.sf.picard.sam.SamToFastq.main(SamToFastq.java:118)
    Last edited by upper; 12-05-2012, 05:33 PM.
  • swbarnes2
    Senior Member
    • May 2008
    • 910

    #2
    Set the VALIDATION_STRINGENCY to LENIENT. Picard will still warn you about those reads, but it will go past them.

    bwa will throw the 4 flag if a read hangs off the edge of one of your references, but it will also give it a mapping position and MAPQ. That likely is the source of your problem reads.

    Comment

    • upper
      Member
      • May 2010
      • 10

      #3
      Originally posted by swbarnes2 View Post
      Set the VALIDATION_STRINGENCY to LENIENT. Picard will still warn you about those reads, but it will go past them.

      bwa will throw the 4 flag if a read hangs off the edge of one of your references, but it will also give it a mapping position and MAPQ. That likely is the source of your problem reads.
      thank you,picard ignore these reads and continue process,and also picard just report error as before but didnot remove these reads,this is what I want.

      Code:
      INPUT=s_3_2/s_3_2_unmap_genome.sam FASTQ=s_3_2/s_3_2_unmap_genome.fq VALIDATION_STRINGENCY=LENIENT
      
      Ignoring SAM validation error due to lenient parsing:
      Error parsing text SAM file. MAPQ should be 0 for unmapped read.; Line 3205583
      Line: HWUSI-EAS724_0039:3:34:15714:3092#0       20      9       124595110       37      80M     *       0       0       AGTTAATGTAGCTTAATAACAAAGCAAAGCACTGAAA
      ATGCTTAGATGGATAATTGTATCCCATAAACACAAAGGTTTGG        hggh]hghhhghghhhhhghhhhhhhhhhhdhhhhhhhcfhhghhhhhhhchhhghhfdhhhghhhhhhgghhghhghhh        XT:A:U  NM:i:1  XN
      :i:1  X0:i:1  X1:i:0  XM:i:1  XO:i:0  XG:i:0  MD:Z:0G79

      Comment

      Latest Articles

      Collapse

      • SEQadmin2
        Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
        by SEQadmin2



        Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

        Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
        ...
        10-14-2026, 09:55 AM
      • SEQadmin2
        New Genomics Technologies Take Aim at Long-Standing Limits
        by SEQadmin2


        Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

        We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
        ...
        09-28-2026, 10:25 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 10-22-2026, 11:13 AM
      0 responses
      49 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-22-2026, 10:48 AM
      0 responses
      37 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-15-2026, 10:52 AM
      0 responses
      46 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-14-2026, 05:39 AM
      0 responses
      46 views
      0 reactions
      Last Post SEQadmin2  
      Working...