biom from QIIME to MEGAN
Greetings all,
I was wondering if someone could help me with an issue importing a .biom file from QIIME into MEGAN. All works well when I import the OTU table (.biom) initially, however it doesn't work after my manual manipulations of the OTU table... here is my process...
1. Create OTU table from pick_otus.py and assign_taxonomy.py
2. Convert the OTU table (.biom) to a tab delimited text file which I manually edited in Excel (some taxonomies were suspect and lacking so I manually edited them based on other BLAST results.
3. I set up the OTU table to look EXACTLY like the old tab delimited one and convert it back to a .biom file but MEGAN will not import any taxonomic stream or OTU quantities.
Any help for formatting this correctly to use in MEGAN would be greatly appreciated. I can provide further details if necessary. Thanks.
I was wondering if someone could help me with an issue importing a .biom file from QIIME into MEGAN. All works well when I import the OTU table (.biom) initially, however it doesn't work after my manual manipulations of the OTU table... here is my process...
1. Create OTU table from pick_otus.py and assign_taxonomy.py
2. Convert the OTU table (.biom) to a tab delimited text file which I manually edited in Excel (some taxonomies were suspect and lacking so I manually edited them based on other BLAST results.
3. I set up the OTU table to look EXACTLY like the old tab delimited one and convert it back to a .biom file but MEGAN will not import any taxonomic stream or OTU quantities.
Any help for formatting this correctly to use in MEGAN would be greatly appreciated. I can provide further details if necessary. Thanks.